Male Fisher 344 rats were purchased in Japan SLC (Shizuoka, Japan). The rat aorta smooth muscle cell line, A7r5, was acquired from DS Pharma Biomedical Co. Limited (Osaka, Japan). A persons cervical cancer cell line, HeLa, and also the human osteosarcoma cell line, HOS, were acquired in the Health Science Research Sources Bank (Osaka, Japan). Cell culture medium was purchased in Sigma-Aldrich (St. Louis, MO). Fetal bovine serum (FBS) was purchased in JRH Biosciences (Lenexa, KS). Antibiotics were purchased in Existence Technologies Japan Limited. (Tokyo, japan, Japan). Other reagents were purchased in Wako Pure Chemical Industries Limited. (Osaka, Japan), Sigma-Aldrich, and Existence Technologies Japan Limited.